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Course info
KFY / 7OSP1
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Course description
Department/Unit / Abbreviation
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KFY
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7OSP1
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Academic Year
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2023/2024
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Academic Year
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2023/2024
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Title
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Optical Spectroscopy 1
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Form of course completion
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Exam
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Form of course completion
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Exam
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Accredited / Credits
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Yes,
4
Cred.
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Type of completion
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Oral
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Type of completion
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Oral
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Time requirements
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lecture
2
[Hours/Week]
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Course credit prior to examination
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No
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Course credit prior to examination
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No
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Automatic acceptance of credit before examination
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No
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Included in study average
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YES
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Language of instruction
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Czech
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Occ/max
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Automatic acceptance of credit before examination
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No
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Summer semester
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1 / -
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0 / -
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0 / -
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Included in study average
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YES
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Winter semester
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27 / -
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0 / -
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1 / -
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Repeated registration
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NO
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Repeated registration
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NO
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Timetable
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Yes
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Semester taught
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Winter semester
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Semester taught
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Winter semester
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Minimum (B + C) students
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not determined
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Optional course |
Yes
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Optional course
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Yes
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Language of instruction
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Czech
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Internship duration
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0
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No. of hours of on-premise lessons |
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Evaluation scale |
A|B|C|D|E|F |
Periodicity |
every year
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Specification periodicity |
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Fundamental theoretical course |
Yes
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Fundamental course |
Yes
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Fundamental theoretical course |
Yes
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Evaluation scale |
A|B|C|D|E|F |
Substituted course
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KFY/OSPP1
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Preclusive courses
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N/A
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Prerequisite courses
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N/A
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Informally recommended courses
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N/A
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Courses depending on this Course
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KFY/OSPC1, KFY/OSPS1, KFY/ZPOSC, KFY/7OSC1, KFY/7OSS1
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Histogram of students' grades over the years:
Graphic PNG
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XLS
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Course objectives:
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Spectral and photometric characteristics of electromagnetic radiation, classification of spectral methods. Interaction of electromagnetic radiation and matter, the physical nature of radiation absorption. Basic scheme of optical spectroscopic apparatus. Methods of measurement of UV-VIS absorption spectra - analytical use. Quantitative description of absorption in the UV-VIS region - Lambert-Beer Law, quantification of mixtures of absorbing substances. Principles and application of Infrared Spectroscopy, Raman Spectroscopy. Physical principles of fluorescence (Jablonski diagram, radiation and non-radiation transitions). Fluorescence of organic molecules, medium influence on spectra. Experimental devices, methods of measurement of fluorescence spectra and their correction.
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Requirements on student
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The oral examination will be rated according to the knowledge within the range of presented issues.
Basic knowledge of optics, quantum physics.
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Content
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1. Properties of electromagnetic radiation, spectral characteristics of radiation, photometric characteristics, classification of spectral methods. 2. Interaction of the matter with electromagnetic radiation, propagation of electromagnetic waves in the environment, elastic and nonelastic interaction of the radiation with the matter. Basic relations of absorption spectroscopy, conditions for radiation absorption, types of electron transitions, influence of intermolecular interactions on electron absorption spectra. 3. Basic scheme of optical spectroscopic instrument, sources of optical radiation. 4. Monochromatization of optical radiation, interferometers, radiation detectors. 5. Intensity of the absorption band, electromagnetic wave attenuation in the absorbing environment, Lambert law, absorption coefficient in the condensed phase, Beer's law. 6. Measurement of absorption spectra, methods based on transmittance measurements (single and double beam absorption spectrophotometer), preparation of samples for the measurement of transmittance, reflection methods, nephelometry and turbidimetry, photoacoustic spectroscopy. 7. Analytical use of UV and VIS spectroscopy, calibration curve, concentrated and highly diluted solutions, determination of concentration in a mixture containing two or more components, spectrophotometric titration, study of chemical equilibrium, use of electron absorption spectroscopy in biophysics. 8. Infrared (IR) spectroscopy. Vibrational, rotational and vibration-rotational spectra of molecules, description of molecule vibrations, selection rules, construction and principle of operation of infrared spectrometers. Samples for IR spectroscopy. Interpretation and use of IR spectra. Dispersion and FT infrared spectrometers. Raman spectroscopy. 9. Definition of luminescence and basic quantities characterizing luminescence. Stokes' law. Basic luminescence classification. Fluorescence characteristics based on transitions between electron vibration levels of complex molecules (Jablonski diagram). Radiation and nonradiation transitions. 10. Spectrum of luminescence of complex organic molecules. Absorption and luminescence conditions in the UV-VIS-NIR spectrum. Types of organic fluorophores (natural and artificial fluorescence probes). 11. Relationships between absorption and fluorescence. Law of mirror symmetry between absorption and fluorescence band. Influence of environment on absorption and fluorescence spectra. 12. Experimental devices for steady-state luminescence spectroscopy, measurement of excitation and emission spectra, measurement of quantum yield of fluorescence. Instrument calibration, geometry of the experiment. Correction of emission and excitation spectra.
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Activities
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Fields of study
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Guarantors and lecturers
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Literature
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Basic:
Atkins P., de Paula J. Fyzikální chemie. VŠCHT Praha, 2013. ISBN 978-80-7080-830-6.
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Basic:
Kalina, J., Špunda, V. Optická spektroskopie I. Ostrava, 2004. ISBN 80-7368-000-9.
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Recommended:
Launay, J.-P.; Verdaguer M. Electrons in Molecules: From Basic Principles to Molecular Electronics.. Oxford University Press, 2013. ISBN 978-0199297788.
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Recommended:
PROSSER V. aj. Experimentální metody biofyziky. Praha: Academia, 1989. ISBN 80-200-0059-3.
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Recommended:
Bishop, D.M. Group Theory and Chemistry.. Dover Publications, 1993. ISBN 978-0486673554.
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Recommended:
Gauglitz, G., Vo-Dinh, T. Handbook of spectroscopy. 2014. ISBN 978-3-527-32150-6.
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Recommended:
Kittel, C. Introduction to Solid State Physics.. Wiley, 2005. ISBN 9780471415268.
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Recommended:
HARRIS D. A. Light spectroscopy.. Oxford: BIOS Scientific Publishers, 1996. ISBN 1-872748-34-1.
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Recommended:
Pelant, I.; Valenta, J. Luminiscenční spektroskopie I. Objemové krystalické polovodiče.. Academia, 2006. ISBN 8020014470.
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Recommended:
Pelant I., Valenta J. Luminiscenční spektroskopie II: Nanostruktury, elektroluminiscence, stimulovaná emise. Academia, 2010. ISBN 978-8020018465.
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Recommended:
Szabo, A.; Ostlund, N.S. Modern Quantum Chemistry: Introduction to Advanced Electronic Structure Theory. Dover Publications, 1996. ISBN 978-0486691862.
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Recommended:
Wilson, E.B.Jr.; Decius, J.C.; Cross P.C. Molecular Vibrations: The Theory of Infrared and Raman Vibrational Spectra.. Dover Publications, 1980. ISBN 978-0486639413.
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Recommended:
Tkachenko, N. Optical Spectroscopy. Methods and Instrumentations.. Elsevier Science, 2006. ISBN 9780444521262.
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Recommended:
Levine, I.N. Quantum Chemistry.. 7th edition, Pearson Education Dorling Kindersley, 2016. ISBN 9789332558533.
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Recommended:
Engel, T. Quantum Chemistry and Spectroscopy.. 3rd. edition, Pearson India, 2012. ISBN 978-9332544956.
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Recommended:
Hammes, G.G. Spectroscopy for the Biological Sciences.. 1st. edition, John Wiley & Sons, Inc., 2005. ISBN 978-0471713449.
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Recommended:
McWeeny, R. Symmetry: An Introduction to Group Theory and Its Applications.. Dover Publications, 2002. ISBN 978-0486421827.
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Recommended:
Harris, D.C., Bertolucci M.D. Symmetry and Spectroscopy: An Introduction to Vibrational and Electronic Spectroscopy.. Dover Publications, 1989. ISBN 978-0486661445.
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On-line library catalogues
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Time requirements
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All forms of study
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Activities
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Time requirements for activity [h]
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Being present in classes
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26
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Self-tutoring
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19
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Consultation of work with the teacher/tutor (incl. electronic)
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10
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Scientific text studying in the Czech language
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20
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Preparation for an exam
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30
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Scientific text studying in a foreign language
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15
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Total
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120
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Prerequisites
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Learning outcomes
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Knowledge - knowledge resulting from the course: |
The student: - knows spectral and photometric characteristics of radiation and types of spectral methods. - understands interaction of electromagnetic radiation with matter, physical principle of radiation absorption. - is familiar with basic scheme of optical spectroscopic apparatus and knows methods of absorption spectra measurement, UV, VIS and IR spectroscopy and its analytical application. - knows quantitative description of absorption in UV-VIS spectral region - Lambert-Beer law, quantification of mixtures of absorbing substances. - acquires basic information about physical principles of fluorescence (Jablonski diagram, radiative and non-radiative transitions). - knows fluorescence of organic molecules (mirror symmetry of absorption and emission band, the influence of environment on spectra). - is familiar with experimental equipment, methods of fluorescence spectra measurement and their correction.
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Assessment methods
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Knowledge - knowledge achieved by taking this course are verified by the following means: |
IC6 - Oral examiantion |
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Teaching methods
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Knowledge - the following training methods are used to achieve the required knowledge: |
A1 - Lecture |
C5 - Static and dynamic projection/presentation |
B2 - Productive work with text/image/educational source |
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