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  Abbreviation unit / Course abbreviation Title Variant
Item shown in detail - course KFY/7OSP1  KFY / 7OSP1 Optical Spectroscopy 1 Show course Optical Spectroscopy 1 2023/2024

Course info KFY / 7OSP1 : Course description

  • Course description , selected item
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Department/Unit / Abbreviation KFY / 7OSP1 Academic Year 2023/2024
Academic Year 2023/2024
Title Optical Spectroscopy 1 Form of course completion Exam
Form of course completion Exam
Accredited / Credits Yes, 4 Cred. Type of completion Oral
Type of completion Oral
Time requirements lecture 2 [Hours/Week] Course credit prior to examination No
Course credit prior to examination No
Automatic acceptance of credit before examination No
Included in study average YES
Language of instruction Czech
Occ/max Status A Status A Status B Status B Status C Status C Automatic acceptance of credit before examination No
Summer semester 1 / - 0 / - 0 / - Included in study average YES
Winter semester 27 / - 0 / - 1 / - Repeated registration NO
Repeated registration NO
Timetable Yes Semester taught Winter semester
Semester taught Winter semester
Minimum (B + C) students not determined Optional course Yes
Optional course Yes
Language of instruction Czech Internship duration 0
No. of hours of on-premise lessons Evaluation scale A|B|C|D|E|F
Periodicity every year
Specification periodicity Fundamental theoretical course Yes
Fundamental course Yes
Fundamental theoretical course Yes
Evaluation scale A|B|C|D|E|F
Substituted course KFY/OSPP1 
Preclusive courses N/A
Prerequisite courses N/A
Informally recommended courses N/A
Courses depending on this Course KFY/OSPC1, KFY/OSPS1, KFY/ZPOSC, KFY/7OSC1, KFY/7OSS1
Histogram of students' grades over the years: Graphic PNG ,  XLS
Course objectives:
Spectral and photometric characteristics of electromagnetic radiation, classification of spectral methods. Interaction of electromagnetic radiation and matter, the physical nature of radiation absorption. Basic scheme of optical spectroscopic apparatus. Methods of measurement of UV-VIS absorption spectra - analytical use. Quantitative description of absorption in the UV-VIS region - Lambert-Beer Law, quantification of mixtures of absorbing substances. Principles and application of Infrared Spectroscopy, Raman Spectroscopy. Physical principles of fluorescence (Jablonski diagram, radiation and non-radiation transitions). Fluorescence of organic molecules, medium influence on spectra. Experimental devices, methods of measurement of fluorescence spectra and their correction.

Requirements on student
The oral examination will be rated according to the knowledge within the range of presented issues.
Basic knowledge of optics, quantum physics.




Content
1. Properties of electromagnetic radiation, spectral characteristics of radiation, photometric characteristics, classification of spectral methods.
2. Interaction of the matter with electromagnetic radiation, propagation of electromagnetic waves in the environment, elastic and nonelastic interaction of the radiation with the matter. Basic relations of absorption spectroscopy, conditions for radiation absorption, types of electron transitions, influence of intermolecular interactions on electron absorption spectra.
3. Basic scheme of optical spectroscopic instrument, sources of optical radiation.
4. Monochromatization of optical radiation, interferometers, radiation detectors.
5. Intensity of the absorption band, electromagnetic wave attenuation in the absorbing environment, Lambert law, absorption coefficient in the condensed phase, Beer's law.
6. Measurement of absorption spectra, methods based on transmittance measurements (single and double beam absorption spectrophotometer), preparation of samples for the measurement of transmittance, reflection methods, nephelometry and turbidimetry, photoacoustic spectroscopy.
7. Analytical use of UV and VIS spectroscopy, calibration curve, concentrated and highly diluted solutions, determination of concentration in a mixture containing two or more components, spectrophotometric titration, study of chemical equilibrium, use of electron absorption spectroscopy in biophysics.
8. Infrared (IR) spectroscopy. Vibrational, rotational and vibration-rotational spectra of molecules, description of molecule vibrations, selection rules, construction and principle of operation of infrared spectrometers. Samples for IR spectroscopy. Interpretation and use of IR spectra. Dispersion and FT infrared spectrometers. Raman spectroscopy.
9. Definition of luminescence and basic quantities characterizing luminescence. Stokes' law. Basic luminescence classification. Fluorescence characteristics based on transitions between electron vibration levels of complex molecules (Jablonski diagram). Radiation and nonradiation transitions.
10. Spectrum of luminescence of complex organic molecules. Absorption and luminescence conditions in the UV-VIS-NIR spectrum. Types of organic fluorophores (natural and artificial fluorescence probes).
11. Relationships between absorption and fluorescence. Law of mirror symmetry between absorption and fluorescence band. Influence of environment on absorption and fluorescence spectra.
12. Experimental devices for steady-state luminescence spectroscopy, measurement of excitation and emission spectra, measurement of quantum yield of fluorescence. Instrument calibration, geometry of the experiment. Correction of emission and excitation spectra.



Activities
Fields of study


Guarantors and lecturers
  • Guarantors: Mgr. Martin Navrátil, Ph.D. (100%), 
  • Lecturer: Mgr. Martin Navrátil, Ph.D. (100%), 
Literature
  • Basic: Atkins P., de Paula J. Fyzikální chemie. VŠCHT Praha, 2013. ISBN 978-80-7080-830-6.
  • Basic: Kalina, J., Špunda, V. Optická spektroskopie I. Ostrava, 2004. ISBN 80-7368-000-9.
  • Recommended: Launay, J.-P.; Verdaguer M. Electrons in Molecules: From Basic Principles to Molecular Electronics.. Oxford University Press, 2013. ISBN 978-0199297788.
  • Recommended: PROSSER V. aj. Experimentální metody biofyziky. Praha: Academia, 1989. ISBN 80-200-0059-3.
  • Recommended: Bishop, D.M. Group Theory and Chemistry.. Dover Publications, 1993. ISBN 978-0486673554.
  • Recommended: Gauglitz, G., Vo-Dinh, T. Handbook of spectroscopy. 2014. ISBN 978-3-527-32150-6.
  • Recommended: Kittel, C. Introduction to Solid State Physics.. Wiley, 2005. ISBN 9780471415268.
  • Recommended: HARRIS D. A. Light spectroscopy.. Oxford: BIOS Scientific Publishers, 1996. ISBN 1-872748-34-1.
  • Recommended: Pelant, I.; Valenta, J. Luminiscenční spektroskopie I. Objemové krystalické polovodiče.. Academia, 2006. ISBN 8020014470.
  • Recommended: Pelant I., Valenta J. Luminiscenční spektroskopie II: Nanostruktury, elektroluminiscence, stimulovaná emise. Academia, 2010. ISBN 978-8020018465.
  • Recommended: Szabo, A.; Ostlund, N.S. Modern Quantum Chemistry: Introduction to Advanced Electronic Structure Theory. Dover Publications, 1996. ISBN 978-0486691862.
  • Recommended: Wilson, E.B.Jr.; Decius, J.C.; Cross P.C. Molecular Vibrations: The Theory of Infrared and Raman Vibrational Spectra.. Dover Publications, 1980. ISBN 978-0486639413.
  • Recommended: Tkachenko, N. Optical Spectroscopy. Methods and Instrumentations.. Elsevier Science, 2006. ISBN 9780444521262.
  • Recommended: Levine, I.N. Quantum Chemistry.. 7th edition, Pearson Education Dorling Kindersley, 2016. ISBN 9789332558533.
  • Recommended: Engel, T. Quantum Chemistry and Spectroscopy.. 3rd. edition, Pearson India, 2012. ISBN 978-9332544956.
  • Recommended: Hammes, G.G. Spectroscopy for the Biological Sciences.. 1st. edition, John Wiley & Sons, Inc., 2005. ISBN 978-0471713449.
  • Recommended: McWeeny, R. Symmetry: An Introduction to Group Theory and Its Applications.. Dover Publications, 2002. ISBN 978-0486421827.
  • Recommended: Harris, D.C., Bertolucci M.D. Symmetry and Spectroscopy: An Introduction to Vibrational and Electronic Spectroscopy.. Dover Publications, 1989. ISBN 978-0486661445.
  • On-line library catalogues
Time requirements
All forms of study
Activities Time requirements for activity [h]
Being present in classes 26
Self-tutoring 19
Consultation of work with the teacher/tutor (incl. electronic) 10
Scientific text studying in the Czech language 20
Preparation for an exam 30
Scientific text studying in a foreign language 15
Total 120

Prerequisites

Learning outcomes

Knowledge - knowledge resulting from the course:
The student:
- knows spectral and photometric characteristics of radiation and types of spectral methods.
- understands interaction of electromagnetic radiation with matter, physical principle of radiation absorption.
- is familiar with basic scheme of optical spectroscopic apparatus and knows methods of absorption spectra measurement, UV, VIS and IR spectroscopy and its analytical application.
- knows quantitative description of absorption in UV-VIS spectral region - Lambert-Beer law, quantification of mixtures of absorbing substances.
- acquires basic information about physical principles of fluorescence (Jablonski diagram, radiative and non-radiative transitions).
- knows fluorescence of organic molecules (mirror symmetry of absorption and emission band, the influence of environment on spectra).
- is familiar with experimental equipment, methods of fluorescence spectra measurement and their correction.

Assessment methods

Knowledge - knowledge achieved by taking this course are verified by the following means:
IC6 - Oral examiantion

Teaching methods

Knowledge - the following training methods are used to achieve the required knowledge:
A1 - Lecture
C5 - Static and dynamic projection/presentation
B2 - Productive work with text/image/educational source
 

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